Navi from Ocarina of Time: HAY HAY HAY HAY HAY LUUUK! LUUK! HAY HAY HAY HAY HAY! WATCH AUGHT! Ugh! Leave me alone... Thankfully, Tatl in Majora's Mask just made dinging noises. Kind of annoying, but not Navi annoying!
Juri from Street Fighter IV and Super Street Fighter IV: Her voice is irritating. Her moves aren't too bad and can be helpful with a little practice, but man. And while we're on the subject...
Rufus from Street Figther IV and Super Street Fighter IV: Not only do his moves suck ass, he never shuts the fuck up. Just never! Plus, he looks like Fat Bastard in a clown suit.
Professor Layton from the Professor Layton series: While he does don a very gentlemanly voice, he always has a damn puzzle up his sleeves and presents them to you in the most inappropriate times. The games were brain rape puzzles complete with annoying music. OK games, trading them in to put towards OOT 3D.
Sheva Alomar (and forgive me if I spelled her name wrong) from Resident Evil 5: An OK game that really isn't as good as RE4, but whatever, not the point. If you thought Ashley from RE4 was a pain in the ass, just wait and play with Sheva... It's like playing with a 6 year old with a cocaine addiction. She looks sexy, but then she opens her mouth and says stupid shit. For instance, when you're fighting Wesker for the second time on the boat, note he can dodge bullets. You figure that out in the first fight you have with him. Of course, Sheva needs to point that out for you. But no, it gets better. As she points that out to you, she continues to shoot at Wesker. But of course, the one flaw that makes her annoying. Her AI. Put her in defend mode, she'll use shitty weapons against big guys and OH, get in your way. Put her in attack mode, she'll use up all the good weapons. So here's what you do: Call your best friend up, get his or her ass over to your house, tell them how to play RE5, and go tell Sheva's AI to go fuck itself!
Howard "Buckshot" Holmes and Kreese Kreeley from Madworld: Now this game is enjoyable. Nothing is better than ripping the living shit out of anyone in your way with your chainsaw. So satisfying! But there are 3 things that kill it (using that generously of course) for me: 1. the iffy controls on the Wii. 2. the length of the game being too short. And 3. Howard "Buckshot" Holmes and Kreese Kreeley themselves. They always talk. And you know what, Kreeley is played by John DiMaggio. The same guy who does Bender on Futurama? Yeah, he's in it and you know what? It doesn't change my opinion. And even worst, you don't have the option to shut those ass holes up. You HAVE to put up with them. They say the same shit all the time over the rapping that plays during game play, the guys you're killing, and the guys attempting to kill you. If your ears could have seizures, they would.
There's probably more, but I'll get back to you all on it.